Fig. 6. Alignment between the amino acid sequences of the coho (Oncorhynchus kisutch, Walbaum) RH2A (Dann et al., 2004) and RH2B protein deduced from coho middle-wavelength-sensitive (MWS) opsin cDNA show 86.1 percent amino acid sequence identity. Conserved residues in positions analogous to bovine I113, C187 and K296 are in bold. The 48 amino acid differences between the RH2A and RH2B are marked by asterisks below the sequence. The E123Q substitution (analogous to position 122 in bovine rod opsin), which may be responsible for the short-wavelength shift of the RH2B relative to RH2A, is in bold with an asterisk below.