Fig. 8. Differences in cupular morphology and frequency response for larvae of different ages. In each box, the center line represents the median value, the upper and lower bounds of the box represent the interquartile range, and the whiskers indicate the total range. Outliers defined as exceeding 1.5 times the interquartile range are denoted by a plus sign. Measurements for (A) cupula height (h_c), (B) kinocilia height (h_k), (C) cupula diameter at kinocilia tips (d_k), (D) cupula diameter at its base (d_b), (E) peak sensitivity and (F) cut-off frequency are presented for multiple individuals. These data are shown for larvae at 3 d.p.f. both (i) prior to (N=5) and (ii) after hatching (N=5), and at (iii) 4 d.p.f. (N=3) and (iv) 5–20 d.p.f. (N=13) using the mean values among neuromasts for individual larvae. The letters (a, b or c) indicate statistical groups as determined by one-way ANOVA with a post-hoc comparison using Tukey's least significant difference procedure such that two ages must not share any statistical groups to be considered statistically different.