Fig. 6. The effect of rearing temperature on the measured protein levels (as indicated by antibody binding) at stage 4 (Gosner stage 42) for the populations studied. On the basis of two-way ANOVA, the effect of temperature was statistically significant for the putative HIF but not for HSP70 or HSP90 after the effect of sampling location was taken into account. Filled bars indicate 13°C, open bars indicate 19°C. SK, Skåne; UP, Uppland; NO, Norrbotten. (A) HIF. For SK N=7 at 13°C and N=8 at 19°C; for UP N=7 and 8, respectively; for NO N=8 and 7, respectively. (B) HSP70. For SK N=9 at 13°C and N=8 at 19°C; for UP N=8 and 10, respectively; and for NO N=8 and 9, respectively. (C) HSP90. For SK N=7 at 13°C and N=8 at 19°C; for UP N=9 and 7, respectively; and for NO N=6 and 8; respectively. Values are means and s.e.m. (indicated as lines above the bars).