Fig. 5. The levels of the three measured proteins at developmental stage 4 (Gosner 42) in the different populations at 13°C (A) and at 19°C (B). SK denotes the southernmost and NO northernmost population (SK, Skåne; UP, Uppland; NO, Norrbotten). At 13°C for HIF N=7 for SK, 7 for UP, and 8 for NO; for Hsp70, N=9, 8 and 8; and for Hsp90 N=7, 9 and 6. At 19°C N=8, 8 and 7 for HIF; N=7, 10 and 9 for HSP70; and N=8, 7 and 8 for HSP90. There was a significant (P<0.01, ANOVA) effect of latitude on all the proteins at 13°C; pairwise comparisons with a post-hoc test (LSD test) indicated that the Skåne population differed significantly (P<0.01) from the other two populations. At 19°C, there was an effect only in HSP90, where the Skåne population again differed (P<0.01) from the other two.