Fig. 2. A schematic representation of HIF-1
protein in Rana
temporaria and other selected vertebrates. The crucial interaction
domains and hydroxylation targets of the molecule are shown. The basic
helix-loop-helix (bHLH) and the Per-ARNT-Sim (PAS) domains are involved in DNA
binding and dimerization of the protein. The oxygen-dependent degradation
(ODD) and the C-terminal transactivation domains (C-TAD) confer
oxygen-dependent regulation. The figure also gives the length of the deduced
HIF-1 protein and a comparison of its insertion/deletion patterns. In
the block diagram, grey represents teleost-specific insertions that are absent
from tetrapods. Black represents protein regions that are present in mammalian
molecules, but to a variable degree absent from those of other vertebrates. In
the schematic representation of the molecules, lines indicate gaps in the
alignment. Accession numbers – Homo sapiens: GenBank,
NM_001530