Fig. 3. Effect of acclimation temperature on proton leak kinetics of isolated liver (A) and skeletal muscle mitochondria (B) of fed cane toads. Experiments were carried out using liver mitochondria of CA (open circles) and WA (filled circles) and skeletal muscle mitochondria of CA (open squares) and WA (filled squares) cane toads. A shift of the proton leak curve to the right indicates a lower proton conductance of CA liver mitochondria (A), while the acclimation temperature has no effect in skeletal muscle mitochondria (B). Values are means ± s.e.m. from eight (CA group) or nine (WA group) independent preparations. ^*P<0.05 (two-way ANOVA).