Fig. 5. Concentrations of (A) sorbitol and glucose, and (B) alanine, betaine, trehalose and mannitol in D. octaedra cocoons. Samples were taken from untreated controls (0), during a gradual pre-acclimation to desiccation stress at 91% RH (G2–G24) or after a 4 day acute exposure to 91% RH (A4). Samples during the gradual pre-acclimation were taken after 2, 4, 8, 14 and 24 days (see Fig. 1). Asterisks indicate significant difference from day 0 (P<0.05). All values are means ± s.e.m.