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Figure 5


Fig. 5. Impact of cytochrome c (cyt-c) addition on the activation of caspase 3 and caspase 9 in cytosolic extracts from (A) human hepatoma cells (C3A) and (B) diapause embryos of Artemia franciscana. Caspase activities were measured following the increase in fluorescence (F; in arbitrary units, a.u.) due to cleavage of Z-LEHD-R110 (caspase 9) or Z-DEVD-R110 (caspase 3). Cyt-c additions were saturating for C3A cells. No activation by cyt-c was observed for A. franciscana extracts (modified from Menze and Hand, 2007).