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Figure 6


Fig. 6. TNT expression early in adult development is required in both Gyc-89Da and Gyc-89Db neurons to block eclosion. (A–C) Expression of dsRed in CNS of p89Da-GAL4;tubP-GAL80ts;UAS-dsRed-expressing developing adults. Animals were raised at 18°C until 96 h after white puparium formation and then transferred to 30°C. After 0 (A), 7 (B) and 16 h (C) the brain was removed and observed under fluorescence optics. Expression of dsRed in the CNS was seen 7 h after transfer to 30°C. (D) Eclosion rates of flies that express TNTa at different times of adult development in the Gyc-89Da (filled symbols) and Gyc-89Db (open symbols) neurons. Larvae containing tubP-GAL80ts and p89Da-GAL4 or p89Db-GAL4 and UAS-TNTa (solid line) or UAS-TNTi (broken line) were raised at 18°C until white puparium formation and then transferred to 30°C at the times shown. The majority of animals transferred early in adult development (24–48 h after white puparium) had a low eclosion rate whereas those transferred after about 100 h showed little difference in their eclosion rates compared with controls.