Fig. 6. to¹ (takeout mutant) defects in sexual dimorphism of locomotor activity are related to juvenile hormone (JH) level. (A) Wild-type flies present a sexual dimorphism in locomotor activity, as the males do not have the same number of start/stop phases compared with females. This sexual dimorphism is absent in to¹. This phenotype is rescued by directed genetic expression of to within the to-producing cells. (B) The normal number of start/stop phases is rescued by local application of methoprene (a JH analog) compared with the vehicle alone (ethanol), suggesting that to serves as a JH carrier. The number in each box represents the number of flies recorded for each genotype. CS, Canton Special; rescue, tim-GAL4/UAS-to in a to¹ genetic background. *P<0.05; **P<0.001. Mean ± s.e.m.