Fig. 8. The inhibition of coelomocyte spreading in response to hypotonic shock is specific to Hsc70 and Hsp70. Coelomocytes exposed to 5 µg ml^–1 of other proteins (lactoglobulin, casein and actin) during hypotonic shock spread normally, while those exposed to Hsp during hypotonic shock were inhibited. This result confirms that inhibition of spreading is Hsp-specific and not a result of the interaction of any protein with the cells during hypotonic shock. Values are means ± s.e.m. N=3 replicate experiments in which at least 100 cells were observed.