Fig. 6. Inhibition by Hsp of the spreading of hypotonically shocked coelomocytes. Phagocytic coelomocytes were exposed to hypotonic shock in the presence or absence of 5 µg ml^–1 recombinant Hsp70 or 5 µg ml^–1 bovine Hsc70. Both Hsps dramatically inhibited hypotonic medium-induced spreading in coelomocytes, increasing the numbers of smaller diameter coelomocytes and decreasing the numbers of larger diameter ones. Significant differences (P<0.05) between Hsc70- or Hsp70-treated cells and controls are indicated by asterisks (determined by a two-factor ANOVA). Values are means ± s.e.m. N=5 replicate experiments in which at least 100 cells were observed.