Fig. 10. The appearance of the actin cytoskeleton in Hsp70-treated coelomocytes. Coelomocytes exposed to hypotonic shock in the presence or absence of Hsp70 were fixed and stained with Texas Red phalloidin to visualize the actin cytoskeleton. The actin in coelomocytes incubated in hypotonic medium polymerized into long filaments that extend to the edges of the large, flattened cells (A–C). Hsp70-treated cells failed to spread or spread only partially and the actin appears as unpolymerized pools in the center of the rounded cells (D–F).