Fig. 5. Davenport diagram illustrating changes in acid–base status (mean ± s.e.m.) over 24 h following an abrupt temperature change from 4 to 22°C. Iso-P_CO2 (in µatm) lines are drawn. The buffer line at time 0 h demonstrates the hemolymph buffering capacity of H. americanus reported by Rose et al. (Rose et al., 1998) (–6.8 CO₂ l^–1 pH^–1 unit). Conversion factor: 1 Torr=133.3 Pa.