Fig. 2. The responses of (A) cardiac output and (B) cardiac stroke volume V_SH to increasing filling pressure P_in, and (C) cardiac power output to increasing output pressure P_out, for the perfused 18°C-acclimated sea bass heart preparation with different concentrations of adrenaline (5–500 nmol l^–1) in the perfusate. Values are means ± s.e.m. for the control and maximal stimulation for fish acclimated to 18°C (N=6). Asterisks indicate a significant difference (P<0.05) between control and maximal stimulation.