Fig. 2. Isometric twitch kinetics as a function of temperature and axial location in leopard and mako shark red muscle. (A) Time to peak force development after stimulus; (B) relaxation time to 50% of peak force. Pairwise comparisons of times at each temperature indicate no significant differences between axial location (P>0.05), except for the mako at 15°C where posterior muscle (hatched bars) appeared to be slower than anterior muscle (*P<0.05). Values shown are means ± s.d. (leopard shark, N=3; mako shark, N=3–6). A, anterior location; P, posterior location.