Fig. 6. (A) The escalation in behavioural responses of late instar Antheraea polyphemus larvae to increasing levels of disturbance. C–R–, neither clicking nor regurgitation; C+R–, clicking only; C–R+, regurgitation only; C+R+, clicking and regurgitation. C+R+ increased between one-, two- and five-pinch trials, whereas C+R– decreased between one-, two- and five-pinch trials. (B) The temporal relationship between clicking and regurgitation. In almost all five-pinch trials, the acoustic signaling occurred before regurgitation. *Significant difference (P<0.001).