Fig. 2. Transfer of mosquito larvae from 30%SW (seawater) to FW (freshwater) results in significant decreases of Na⁺, Cl^– and H⁺ concentrations in the hemolymph within 6 h of transfer. Groups of mosquito larvae that were reared in FW were transferred and held in 30%SW for at least 24 h. Larvae were subsequently transferred back to FW at time=0, where they were held for varying periods of time prior to the collection of hemolymph. Ion-selective microelectrodes were used to measure the Na⁺ (A), Cl^– (B), K⁺ (C) and H⁺ (D) ion concentrations of the hemolymph. Values are means ± s.e.m., N=6–14. An asterisk denotes a significant difference from value at time=0.