Fig. 1. Transfer of mosquito larvae from freshwater (FW) to 30%SW (seawater) leads to significant increases in Na⁺, Cl^– and H⁺ concentrations in the hemolymph within 5 h of transfer. Groups of mosquito larvae were transferred from FW to 30%SW at time=0, where they were held for varying periods of time prior to the collection of hemolymph. Ion-selective microelectrodes were used to measure the Na⁺ (A), Cl^– (B), K⁺ (C) and H⁺ (D) ion concentrations of the hemolymph. Values are means ± s.e.m., N=16 for time=0 and N=10 for all other groups. An asterisk denotes a significant difference from value at time=0.