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Figure 7


Fig. 7. Larval fat cells persist in aged adults when cell death is blocked. (A) In situ fat-cell number from Lsp2-Gal4::UAS-GFP/UAS-diap1 adult females in which cell death is blocked (N=15–20 individuals per time point, filled bar) compared with Lsp2-Gal4::UAS-GFP control adult females (N=10–46 individuals per time point, open bar). (Mean initial cell number for Lsp2-Gal4::UAS-GFP/UAS-diap1 was 792 cells; for Lsp2-Gal4::UAS-GFP it was 724 cells.) (B) GFP-fluorescence of Lsp2-Gal4::UAS-GFP/UAS-diap1 adult females (N=15–25 per time point, filled bar). Lsp2-Gal4::UAS-GFP control adult females (N=10–60 individuals per time point, open bar). (Mean initial fluorescence for Lsp2-Gal4::UAS-GFP/UAS-diap1 was 25 600 pixels; for Lsp2-Gal4::UAS-GFP it was 21 900 pixels.) Note, perdurance of GFP-fluorescence does not reflect fat-cell number in the cell death-blocked animals. This is probably because of a loss of activity from the Lsp2 promoter (see Discussion for details). Values are means ± s.d.