Fig. 1. Spatiotemporal maps (STMap) of the luminal flow were constructed from unfed larvae at 4 and 7 d.p.f. (A) A box was drawn around the area of interest, covering most of the gut (900 µm). PI, proximal intestine (intestinal bulb); MI, middle intestine; DI, distal intestine. (B) STMaps, over the area within the box in A, were created based on changes in gut opacity as the gut content was propelled in a retrograde or anterograde direction. Individual contractions are identified as darker bands, travelling in either direction over time (downwards in map, 3.8 min). In this STMap, six anterograde contractions waves are present. Velocity (v, the slope of the line), interval between consecutive contractions (t), distance (d) travelled by the contractions and the total time of activity were calculated. Asterisks indicate the region where the contraction waves originated.