Fig. 6. Dry mass and nutrient reserves in groups of 36 h old fourth instar A. aegypti given different periods of time to feed or not. Newly molted fourth instars were either deprived of food for 36 h, or fed for 12 h only, or fed for 36 h prior to weighing and analyses. (A) Dry mass of fourth instars, (B) lipid amounts in fourth instars, (C) glycogen amounts in fourth instars, and (D) protein levels in fourth instars. All values represent least square means ± s.e.m.; N=12. All nutrient values were obtained from an ANCOVA model and are corrected for larval dry mass (covariate). Error bars represent s.e.m.; no bar indicates that the s.e.m. is smaller than column scale.