Fig. 5. Hemolymph ecdysteroid titers in a single larva (striped bars) and
ecdysteroid secretion by a single set of thorax and abdomen (gray bars) in
groups of 36 h old fourth instar A. aegypti given different periods
of time to feed or not. Newly molted fourth instars were either deprived of
food for 36 h, or fed for 12 h or 36 h prior to tissue collection at 36 h for
the ecdysteroid bioassay. Values represent least square means ± s.e.m.;
N=12. For both ecdysteroid secretion and hemolymph values, columns
with different letters are significantly different from each other
(Tukey–Kramer HSD, P
0.05). NM, newly molted fourth instars
never given access to food. No bar indicates that the s.e.m. is smaller than
column scale.