Fig. 3. (A) Effect of duration of food access on metamorphosis of A. aegypti fourth instars. Shown are percentages of individuals that remained as fourth instars (black bars) or that underwent metamorphosis and subsequently emerged as males (striped bars) or females (gray bars) in response to a period of access to food. Control (C) group of larvae were given food in excess and never underwent a period of time in which food was withheld. N=335. (B) Effect of supplementing food to fourth instars initially fed for 12 h followed by a 7 day fast. Shown are percentages of individuals that remained as fourth instars (black bars) or underwent metamorphosis and subsequently emerged as males (striped bars) or females (gray bars) in response to supplementary feeding. N=80.