Fig. 9. Differences between PGI genotypes in effects of two 4 h temperature treatments on Hsp70 expression in adult beetles. Treatment temperatures, in order, are shown in each panel. Data are least square means (±s.e.m.) of Hsp70 expression (ng of Hsp70 per g of thoracic muscle), measured by western blot analysis of PGI 1-1, 1-4 and 4-4 genotypes [left panels (–4°C second treatment): 20°C: N=15, 10, 2; –4°C: N=17, 9, 2; 36°C: N=15, 10, 2. Right panels (36°C second treatment): 20°C: N=10, 18, 3; –4°C: N=15, 9, 2; 36°C: N=15, 11, 1]. Statistical analysis was performed on Hsp70 data using ANOVA (Table 1).