Fig. 8. Differences in adult and larval running speed measured after first and second temperature treatments. Data are least square means (±s.e.m.) of running speed for the three main PGI genotypes, reported as a function of second temperature treatment [–4°C (open bars), 36 or 35°C (shaded bars)]. Sample sizes are as follows. Adults: –4°C second treatment: N=85, 72, 9; 36°C second treatment: N=73, 79, 23. Larvae: –4°C second treatment: N=39, 52, 14; 35°C second treatment: N=48, 48, 15. Statistical analyses of difference in running speed between two temperature treatments were performed using ANOVA (adults) and ANCOVA (larvae) and results are described in the text and in the supplemental tables (supplementary material Tables S4, S5).