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Figure 2


Fig. 2. Construction and expression of rhodopsin::GFP. (A) A diagram of the DNA fragment that contained 1.2 kb of zebrafish rhodopsin promoter and 0.7 kb of pd2EGFP cDNA with a short poly(A) (pA) tail. (B) Rhodopsin promoter-driven GFP expression in rod photoreceptor cells in the ventral patch of the retina (arrows) at 4 days post-fertilization. Scale bar, 40 µm. (C) Germline transmission of the transgene determined by PCR. Lane 1, molecular markers. Lane 2, positive control (plasmid DNA that contained 0.7 kb of pd2EGFP cDNA). Lanes 3–11, PCR of genomic DNA from nine embryos that were selected from a cross between a transgenic and a wild-type fish. Lanes 3–6, non-transgenic siblings. Lanes 7–11, transgenic siblings. Each lane represents PCR from an individual embryo. Wnt (internal control) was detected in every embryo.