Fig. 8. Absence of caspase-3 activation by CuCl₂, at 25°C or at 42°C, in the absence or presence of CAT and SOD. Immunoblot analysis using a specific antibody that recognizes both the inactive full-length pro-caspase-3 and the fragmented active form of the protein led to the detection of only pro-caspase-3 in samples (100 µg protein) from hearts perfused without or with 500 µmol l^–1 CuCl₂ for 15 min, either in the absence or the presence of 30 U ml^–1 SOD, 150 U ml^–1 CAT or the combination of SOD+CAT, at 25°C or at 42°C. Western blot shown is representative of three independent experiments performed with similar results. The positions of marker proteins are shown on the left.