Fig. 7. Effect of different antioxidants on the p38-MAPK phosphorylation induced by CuCl₂ in the absence or presence of hyperthermia (42°C). Phospho-p38-MAPK was detected in extracts (50 µg protein) from control hearts (Con), hearts perfused for 15 min with 30 U ml^–1 SOD alone or with 500 µmol l^–1 CuCl₂, either in the absence or the presence of 30 U ml^–1 SOD, 150 U ml^–1 CAT or the combination of SOD+CAT, at 25°C. In addition, hearts were perfused for 15 min with 500 µmol l^–1 CuCl₂ and identical combinations of antioxidant agents but at 42°C (Ai). Actin protein levels of identical samples were detected so as to confirm equal protein loading (Aii). Densitometric analysis of phospho-p38-MAPK bands by laser scanning was performed (B). Results are means ± s.e.m. for three independent experiments. ^*P<0.05, ^**P<0.01, P<0.001 vs control value.