Fig. 3. Effect of the selective inhibitor SB203580 on the p38-MAPK and Hsp27 phosphorylation induced by CuCl₂. Protein (50 µg, top and bottom, and 100 µg, middle) from hearts perfused without or with 500 µmol l^–1 CuCl₂ for 15 min in the absence or presence of 1 µmol l^–1 SB203580 was analysed by immunoblotting with phosphospecific anti-p38-MAPK (top), phosphospecific anti-Hsp27 (middle) or anti-actin (bottom) antibodies. Western blots shown are representative of four independent experiments performed with similar results.