Fig. 7. The effect of the generic protein kinase inhibitor H-7 and the PKG inhibitor KT-5823 on the digging rhythm. (Ai) A 10 min bath application of 0.1 mmol l^–1 H-7 resulted in a significant decrease in cycle frequency (t=4.93, ^*P<0.05, mean ± s.e.m. from five animals). A 30 min wash with locust saline resulted in a partial recovery of the digging rhythm to control levels. (Aii) A 10 min bath application of H-7 resulted in a decrease in the number of cycles (three cycles) compared with control (five cycles). A 30 min wash with locust saline resulted in a partial recovery of the digging rhythm to control levels. (Bi) A 10 min bath application of 10 µmol l^–1 KT-5823 resulted in a significant decrease in the cycle frequency (t=2.8, ^*P<0.05, mean ± s.e.m. from five animals). (Bii) A 10 min bath application of KT-5823 resulted in a decrease in the number of cycles (five cycles) from a control value of six. This particular recording shows that a 30 min wash with locust saline did not result in the number of cycles returning to its control value.