Fig. 8. Histogram showing frequency distribution of distances between any two gold particles (corresponding to the location of the D2 antibody) on the same thick filament, as seen by electron microscopy, and the statistical significance analysis. (A) Frequency was set up for a total of 155 measurements of not more than 400 nm and analyzed on the assumption of compound normal distributions with parameters indicated by the maximum likelihood method. (B) Estimated slope (36.29) of the regression line through the origin, compared to 36.25 (see text for details). The two values were not statistically different (F-test, P=0.91).