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Figure 2


Fig. 2. Effects of twitchin D2 peptide mutants on actomyosin Mg2+-ATPase activity. (A) ABRM actomyosin Mg2+-ATPase activity in the presence of 10–4 mol l–1 Ca2+. (B) ABRM actomyosin Mg2+-ATPase activity in the absence of Ca2+. (C) Chicken fast skeletal actomyosin Mg2+-ATPase activity in the presence of 10–4 mol l–1 Ca2+. (D) Actin-activated Mg2+-ATPase activity measured with chicken fast muscle myosin plus chicken F-actin and 10–4 mol l–1 Ca2+. (E) Chicken fast skeletal actomyosin Mg2+-ATPase activity in the presence of 10–4 mol l–1 Ca2+ and 0.4x10–6 mol l–1 ABRM paramyosin. See Materials and methods for details. Values shown are means ± s.d. Numbers above columns are N values. D and A mutants of the TWD2-S are indicated; here TWD2-S is unphosphorylated and Thio-TWD2-S is thiophosphorylated.