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Figure 1


Fig. 1. A schematic representation of the ABRM twitchin molecule and mutants of the D2 peptide. (A) The motif structure of ABRM twitchin is shown together with the region expressed as various 6xHis-fusion proteins that are used in the present study. The D1 and D2 sites are S1075 and S4316, respectively. (B) Phosphorylation of twitchin D2 peptide mutants by PKA. TWD2-S was phosphorylated, whereas TWD2-A and TWD2-D were not phosphorylated. Phosphorylation of TWD2-S was detected by SDS-gel electrophoresis as described previously (Funabara et al., 2001a).