Fig. 6. ClustalW alignment of (A) the N-terminal silk domain and (B) histidine-rich domain of preCol D from M. californianus (Mc), M. edulis (Me) and M. galloprovincialis (Mg). Silk alignment reveals that M. californianus has two major inserts consisting of polyalanine runs in the N terminus. His-rich domain alignment reveals that while overall the identity between M. californianus and the other species is low, the regions containing histidine are more conserved. Residues in bold type are conserved between all three species.