Fig. 10. Micrographs of sections from smolt and adult retinas following double label
in situ hybridization with the green (rtGR) and red (rtRE) riboprobes
or with the UV (rtUV) and blue (rtBL) riboprobes. (A) Tangential section from
the centro-temporal retina of the smolt showing that each member of a double
cone expresses a mRNA encoding green opsin (blue colour) or red opsin (red
colour), and that these alternate around the unit mosaic. (B) Tangential
section from the centro-dorsal retina of the smolt, adjacent to the embryonic
fissure, shows two faint UV riboprobe labels (though no associated cone
morphology is clearly discernable) among the single cone population. (C,D)
Tangential sections from the centro-dorsal (C) and centro-temporal (D) retina
of adult rainbow trout showing the same labelling pattern of double cones as
in the smolt retina. Symbols and nomenclature as in
Fig. 5. Magnification bar (in
A) is 25 µm and relates to all panels.