Fig. 2. HA activates a Cl^– conductance in X-organ neurons. (A) Current-clamp recordings from an X-organ neuron in culture. Note that during the application of HA, the membrane potential (E_m) reached –60 mV at all holding potentials explored. (B,C) HA-evoked currents (I_HA) obtained at different holding potentials from –80 to –20 mV; the interval between each HA pulse was 3 min. All the traces were obtained from the same neuron at two Cl^– equilibrium potentials (–62.5 mV for B and –32 mV for C). (D) Current–voltage relationship for the experimental conditions described in B and C. Both the solid circle and open circle curves correspond to the average ± s.e.m. of 12 cells, and the Cl^– equilibrium potential corresponded to –62.5 mV or –32 mV, respectively.