Fig. 5. A Western blot was used to determine AgCA9 protein localization in larval alimentary canal regions including GC, AMG, PMG, MT and rectum. The left panel of the figure is the Fast Green staining of total protein to ensure equal loading. The strongest AgCA9 signal was seen in both the GC and rectum. CA protein expression was less abundant in the MT and minimal in the AMG and PMG. AgCA9 is indicated by an arrow. For abbreviations, see Fig. 2 legend.