Fig. 3. In situ hybridization was used to detect mRNA in whole larval alimentary canals and carcass. A full-length DIG-labeled RNA probe was used to detect AgCA9 mRNA in whole-mount larvae. Antisense probe generated intense staining in the GC, the transitional region between the anterior and posterior midguts (indicated by arrow) and the rectum of the larval alimentary canal, with weaker staining seen in the cells of the PMG (A). Carcass showed the most intense staining in the muscle fibers and fat body (D). Sense probe did not significantly stain any areas of the alimentary canal (B) or carcass (C). For abbreviations, see Fig. 2 legend. Scale bars: 400 µm.