Fig. 2. qPCR was used to measure expression of AgCA9 mRNA in alimentary canal regions – gastric caeca (GC), anterior midgut (AMG), posterior midgut (PMG), Malpighian tubules (MT) and rectum – relative to whole alimentary canal. All values were normalized to an 18s RNA endogenous control. `Whole' was artificially set to a value of 1.0 in this graph, with all other samples expressed as a proportion of `whole'. Results indicate that AgCA9 mRNA is significantly more abundant in the rectum samples (^*P<0.001 as determined by one-way analysis of variance). Error bars indicate standard deviation between three individual qPCR runs.