Fig. 8. Immunolocalization of AgNHA1 in mosquito midgut paraffin sections. (A) Vesicular staining pattern in the anterior midgut cells (AMG). In numerous sections the staining pattern appeared to be in line with the nucleus of the cell or just apical of the nuclei. (B) Apical localization of AgNHA1 in the posterior midgut cells. (C,D) Sections of AMG and PMG showing the lack of immunostaining by pre-immunization serum. (E,F) Sections in which the antibody was blocked by the AgNHA1 peptide; there was no specific staining other than background.