Fig. 5. The transcription patterns of AgNHA1 in tissues of fourth instar larval An. gambiae. AgNHA1 was detected by in situ hybridization using in vitro transcribed DIG-labeled antisense RNA of AgNHA1. Specific labeling appears purple or dark blue in color. The DIG-labeled sense control showed no specific staining (A). Expression of AgNHA1 transcript was observed in the gastric caeca, GC (B,C), posterior midgut (B,D) and the rectum (B,D). AgNHA1 transcript expression was also observed in the various ganglia of the ventral nerve cord (VNC). AgNHA1 was also expressed in the caudal ganglion (E), the abdominal ganglia (F) and the trilobed thoracic a.k.a. subesophageal ganglia (G). Scale bars, 500 µm (A–D); 75 µm (E–G).