Fig. 8. Co-immunoprecipitation of CTLP1 and CHS2. For immunoprecipitation, cell lysates of the anterior midgut were incubated with the indicated precipitating antibodies (P) and then bound to protein-G–agarose. Unbound proteins were washed away, then bound proteins were eluted, separated by SDS-PAGE and analysed by immunoblotting using detecting antibodies (D) to CTLP1, CHS2 and V-ATPase subunit A (V₁A). Lanes 1–4, co-immunoprecipitation of CHS2 using anti-CTLP1 antibodies (lanes 1,2) and of CTLP1 using anti-CHS antibodies (lanes 3,4); lanes 5–8, control reactions in the absence of precipitating (lanes 5,6) or detecting antibodies (lanes 7,8); lanes 9,10, control reactions for non-specific precipitation of V-ATPase. As a positive control, midgut cell lysates were used (lane 11).