Fig. 5. Localization of CTLP1 and CHS2 mRNAs in the anterior midgut of M. sexta. In situ hybridization was performed under high stringency conditions using 20 µm cryosections of the anterior midgut and antisense RNA probes to detect the sense RNAs for CTLP1 and CHS2 (left). As a negative control, sense RNA probes were use to detect the corresponding antisense RNAs (right).