Fig. 4. Ance is transcribed in the secondary cells of the male accessory gland (AG). In situ hybridisation using digoxigenin-labelled Ance antisense riboprobe (A,C) and sense strand control (B,D) reveals higher levels of mRNA in the large secondary cells (arrow points to one of several secondary cells in the field of view). C and D are higher magnification images of secondary cells and AG lumen. Powerpoint was used to manipulate all the images equally to increase contrast. Scale bars, 10 µm.