Fig. 6. Variation in for affects response to food quality. (A) Expression
of forT2 was sufficient to increase developmental rate and
survivorship in food-deprived sitter larvae raised on low nutrient media (25%
or 15%) [development time: at 25% (F(2,27)=17.78,
P<0.0001) and at 100% (F(2,26)=0.95,
P=0.4); survivorship: at 15% (F(2,29)=4.07,
P<0.03) and at 100% (F(2,28)=0.66,
P=0.5)]. (B) PKG enzyme activity is inversely related to food intake.
Rovers (forR) had significantly higher PKG activity than
sitters (fors and fors2) when reared
on high food levels, but this difference diminished when larvae were
food-deprived [two-way ANOVA: for strain (F(2,63)=57.36,
P<0.0001), food level (F(2,63)=95.26,
P<0.0001), strain-by-food level
(F(4,63)=11.67, P<0.0001); ANOVA: for 100%
(F(2,21)=87.9, P<0.0001), 25%
(F(2,21)=14.39, P<0.0001), 15%
(F(2,21)=0.70, P=0.5) food level]. (C) Sitter
larvae expressing a forT2 transgene expressed a rover-like pattern of
food [two-way ANOVA: for strain (F(2,261)=3.78,
P=0.02), food level (F(2,261)=2.85,
P=0.06), strain-by-food level (F(4,261)=1.60,
P=0.2); one-way ANOVA by strain: for 100%
(F(2,87)=6.87, P<0.002), 25%
(F(2,87)=0.32, P=0.7), 15%
(F(2,87)=0.42, P=0.7) food level].