Fig. 5. Variation in for mediates nutrient acquisition strategies. (A) for^s;hs-GAL4/UASforT2 flies reared at 23°C show an increase in T2 protein level (lane 1) compared to control flies reared under the same conditions (lanes 2 and 3). The actin bands show that equal amounts of protein were loaded in each lane. (B) Expression of a forT2 transgene in sitters resulted in a rover-like decrease in food intake (ANOVA: at 5 min, F_(1,57)=3.03, P=0.09; 10 min, F_(1,58)=7.60, P=0.008; 15 min, F_(1,58)=11.92, P=0.001; 20 min, F_(1,57)=5.46, P=0.02). (C) Ubiquitous expression of forT2 in sitters using a leaky hs-GAL4 transgene significantly decreased food intake (F_(2,89)=19.56, P<0.0001) and (D) increased glucose absorption rate (F_(2,17)=47.51, P<0.0001).