Fig. 6. In vitro confirmation of the effects of 20E on
ecdysteroid-responsive genes. Real-time RT-PCR relative quantification of two
putative ecdysteroid-responsive genes, digestive cysteine proteinase
1 (A) and trypsin (B) in a 24 h hepatopancreatic tissue culture
subjected to four different concentrations of 20E (1, 10, 100 and 1000 pg
µl–1). Different letters indicate statistical significance
(P<0.05).