Fig. 5. Gene expression patterns resulting from the two endocrine manipulations out of all the sequenced and assembled genes from the chip, and the gene ontology (GO) pie graph of the putative ecdysteroid-responsive genes. (A) Schematic overview of the experiment showing the overlap between the two manipulations (XO–SG and 20E), which forms the ecdysteroid-responsive group. Upward-pointing arrows indicate upregulated genes; downward-pointing arrows indicate downregulated genes; `unique sequences' are all the sequenced and clustered genes from the chip. In cases where different clones (spots) from the same gene belonged to different sections of the diagram, these genes were counted multiple times. (B) Pie graph of GO of the putative ecdysteroid-responsive genes. These genes showed a significant response during premolt when ecdysteroid titers were elevated in both treatments.