Fig. 4. Behavior of cells entering and leaving biotin solutions. Cells were analyzed using Motion Analysis as they entered 1 mmol l^-1 biotin in buffer from a control buffer or vice versa. Cells were monitored for turns (% change in direction, PDC) and speed. (A) Cell turning upon entering biotin (On PDC); (B) the T-maze values for the wild type and mutants in biotin for comparison with A because the T-maze values correlate well with On-PDC responses; (C) Cell turning upon leaving biotin (Off PDC). (A) Cam 1-1 and wild type in Na-biotin On PDCs were significantly different from Controls (P<0.05). (B) All Values are means ± 1 s.e.m. of 45 or more measurements. Mann-Whitney U-test was used for statistical analysis of T-maze data; all values except for Cam 11 were different from wild type in Na⁺ and Mg²⁺ at P<0.05 level. (C) Wild type in Mg-biotin and Cam1-1 Off PDCs were significantly different from Controls. The wild type cells were analyzed in K-biotin pH 6.7, Na-biotin pH 7, Mg-biotin pH 7; Cam 1-1 in Na-biotin pH 7; Cam 11 K-biotin pH 6.7; XntA in Na-biotin pH 7 and Mg-biotin pH 7.